Standard syllabus
Bioorganic chemistry · Graduate · Chemistry
Topics
Peptide and protein chemistry
- Solid-phase peptide synthesis: Fmoc and Boc strategies
- Protecting group chemistry for amino acid side chains
- Native chemical ligation and expressed protein ligation
- Protein folding thermodynamics and kinetics
- Unnatural amino acids and genetic code expansion
- Post-translational modifications: glycosylation, phosphorylation, ubiquitination
- Chemical probes for protein function
- Activity-based protein profiling (ABPP)
- Stapled peptides and peptide mimetics
- Proteomics sample preparation and mass spectrometry
Nucleic acid chemistry
- Phosphoramidite DNA/RNA synthesis
- Modified nucleotides for therapeutics and diagnostics
- CRISPR chemistry: guide RNA and Cas9 mechanism
- Antisense oligonucleotides and siRNA
- DNA damage and repair chemistry
- G-quadruplexes and non-canonical structures
- Click chemistry for nucleic acid labeling
- Ribozymes and deoxyribozymes
- Aptamer selection (SELEX) and applications
- Epigenetic modifications: 5-methylcytosine, hydroxymethylation
Enzyme mechanism and inhibition
- Active site architecture and catalytic residues
- Covalent vs non-covalent enzyme inhibition
- Mechanism-based (suicide) inhibitors
- Transition state analog inhibitors
- Allosteric regulation and cooperativity models
- Cofactor chemistry: PLP, thiamine pyrophosphate, biotin
- Radical SAM enzymes and radical mechanisms
- Metalloenzyme mechanisms: zinc, iron, copper enzymes
- Kinase inhibitors in drug discovery
- Protease inhibitors: HIV protease, caspases
Chemical biology tools
- Bioorthogonal chemistry: click reactions in cells
- Photopharmacology and caged compounds
- Fluorescent probes and biosensors
- Chemogenomics and target identification
- Metabolic labeling and flux analysis
- Glycobiology: glycan synthesis and profiling
- Lipidomics and lipid chemical biology
- Chemical inducers of dimerization
- DNA-encoded libraries for screening
- Ethical considerations in chemical biology research
Pricing
Graduate-level rates are set on consultation. See the pricing page for K–12 and undergraduate rates.