Standard syllabus
Advanced spectroscopy · Graduate · Chemistry
Topics
NMR spectroscopy
- Pulse sequences: 90°, 180°, INEPT, DEPT
- Two-dimensional NMR: COSY, TOCSY, NOESY, ROESY
- HSQC, HMQC, HMBC for heteronuclear correlation
- NOE and distance constraints for structure determination
- Dynamic NMR and exchange phenomena
- Solid-state NMR: MAS, CP/MAS, DQMAS
- NMR of paramagnetic systems
- Quantitative NMR (qNMR) for purity and kinetics
- NMR instrumentation: shimming, tuning, cryoprobes
- Structure elucidation workflows for complex molecules
Mass spectrometry
- Ionization methods: EI, CI, ESI, MALDI, APCI, APPI
- Mass analyzers: quadrupole, TOF, ion trap, Orbitrap, FT-ICR
- Tandem MS: CID, ETD, HCD fragmentation
- Accurate mass and isotope pattern analysis
- LC-MS/MS method development and quantitation
- Imaging mass spectrometry (MALDI imaging)
- Ion mobility spectrometry and CCS
- Proteomics and metabolomics MS workflows
- Structural MS: hydrogen/deuterium exchange, cross-linking
- Data analysis: Mascot, Proteome Discoverer, MZmine
Optical and vibrational spectroscopy
- High-resolution laser spectroscopy
- Cavity ring-down and cavity-enhanced absorption
- Coherent anti-Stokes Raman scattering (CARS)
- Surface-enhanced Raman spectroscopy (SERS)
- Time-resolved fluorescence and FRET
- Circular dichroism for biomolecular structure
- Vibrational circular dichroism (VCD)
- Photoelectron spectroscopy and UPS
- X-ray absorption spectroscopy (XAS/XANES/EXAFS)
- Ultrafast spectroscopy: pump–probe, 2D electronic
Magnetic resonance and advanced methods
- EPR/ESR: g-tensors, hyperfine coupling, ENDOR
- Dynamic nuclear polarization (DNP) NMR
- MRI principles and contrast agent chemistry
- Mössbauer spectroscopy for iron compounds
- Muon spin spectroscopy (overview)
- Neutron scattering for hydrogen positions
- Inelastic neutron scattering for vibrational modes
- Combination of spectroscopic methods for structure proof
- Computational prediction and assignment of spectra
- Spectroscopy in operando and in situ studies
Pricing
Graduate-level rates are set on consultation. See the pricing page for K–12 and undergraduate rates.